Nowadays using the combination of green manure and other organic fertilizers intensify the activity of antagonistic microorganisms of parasitic nematodes as well as providing a good substrate to feed soil microphone and micro flora and is useful for large-scale management of plant parasitic nematodes. Tea root lesion nematode, Pratylenchus loosi, is the most important damage-causing factor of tea in Iran and all over the world. Present study aimed to investigate the nematicide organic modifiers efficiency in two levels as infected with the disease agent with a population above the economic threshold (average winter initial population is more than 100 nematodes per 100 g soil) and infected with the disease agent with a population lower than the economic threshold (average winter initial population is less than 80 nematodes per 100 g soil) with four treatments (Neemarin with the dose of 20 per 1000, olive meal and Tobacco wastes both as 7 g per one kg soil and control treatment) in three replicates that was conducted in a Completely Randomized Bulk Design (CRB) in Fashalam tea research station (Shaft). Qualitative indices such as solid content (%), moisture content (%), tannin content (%) and tea water extract (%) and quantitative indices such as nematode population in 100 g soil and one g root and also green leaf yield (kg per ha) were measured. Results related to the infected zone with the disease agent with a population above the economic threshold, showed that Tobacco waste with 71.93% was identified as the best treatment in moisture content index, while in the infected zone with the disease agent with a population lower than the economic threshold, Neemarin with 21.26%, 78.72% and 39.4% were the best treatments in solid content, moisture content and tea water extract indices, respectively. In other qualitative indices, no significant superiority was observed between treatments. In quantitative indices such as nematode population in soil and root, Neemarin and Tobacco wastes were the most important treatments in infected zone with the disease agent with a population above the economic threshold and the disease agent with a population lower than the economic threshold, respectively. For green leaf yield index, there was no significant difference between treatments, but an increase in yield was observed compared to control treatment. Results revealed that using Neemarin and Tobacco wastes can improve qualitative indices as well as reducing nematode population in soil and root and can be used as an important biological agent in tea root lesion nematode control.