Using nematode-resistant cultivars is the most economical and environmentally friendly management strategy which are known for causing great yield losses. For evaluating the reaction of some soybean cultivars to the target nematode, infected roots collected from Isfahan province, then the nematode mass was cultured around cultivar Ps the roots of tomato. Identified with the help of morphological methods, perineal pattern of adult females as well as molecular markers, amplification of 670 bp band of extracted DNA from egg and larvae using single egg mass culture. For evaluating the reaction of soybean cultivars includes sahar, Elit, Williams, 033, L-17, DPX and JK randomized design with five replications. Soybean plants inoculated with 8000 eggs of M. javanica per 2.5 kg of soil. After 80 days the growth and physiologic factors of host plant was measured such as root length, root fresh and dried weight, shoot length, shoot fresh and dried weight, pod number in plant, seed per pod, leaf area index (LAI) and leaf area ratio (LAR) as well as gall index, egg mass and reproductive factors of nematode measured. Then to evaluate the resistance of cultivars, a system based on two factors includes nematode reproductive and root infestation was used. Results showed that the JK cultivar has the most final population of nematodes and Sahar cultivar least final population of nematodes. Results also showed that JK cultivar was sensitive with 2.65 gall index and Elit and Williams cultivars were tolerant and showed less gall index (respectively 0.9 and 1.3) and Sahar, L- 17, 033 and DPX were recognized as resistant. In fact, gall and reproduction they are a very important scales for determine which cultivar has the highest or least resistance to root- knot nematodes. The results showed that JK cultivar was sensitive and Elit and Williams cultivars were tolerance and four cultivars 033, L-17, DPX and Sahar were resistant.