The spider mite, Tetranychus turkestani Ugarov & Nikolski is one of the main pests of crops in Bushehr province and the other southern provinces. Due to the undesirable effects of using chemical pesticides, this research was performed to find alternative safe compounds to environment. Therefore, in this study, fumigant toxicity of three essential oils including Rosmarinus officinalis L., Mentha longifolia L and Eucalyptus globulus Labill. and contact toxicity of two biopesticides tondexir and palizin on mentioned mite female was investigated and compared to spiromesifen as a chemical acaricide. Also, sublethal effects of tested compounds at 50% lethal concentration (LC50) on biological parameters of T. turkestani including adult longevity, fecundity and hatchability were investigated. The experiments were performed at 27  1 oC, 70  5% RH, and a photoperiod of 16:8 (L: D) h. The mean values of LC50 for E. globulus, R. officinalis and M. longifolia were 12.53, 11.47 and 4 μl/l and for spiromesifen, TON and PAL were 10.98, 327.34 and 858.13 ppm, respectively. All tested compounds significantly reduced adult female longevity and no difference was observed between the chemical acaricide spiromesifen and studied plant compounds. Fecundity also reduced in all studied treatments and this reduction was even higher for plant essential oils than for other compounds. Palizin, E. globulus and M. longifolia significantly reduced the egg hatching of T. turkestani like spiromesifen. According to these results, tested plant compounds are effective against T. turkestani and may be applied as safe compounds for crop protection against this pest.