The two-spotted spider mite, Tetranychus urticae Koch has a global distribution and it is one of the most polyphagous known pests of agricultural products of the world. Since the study of the biological parameters of a pest on different varieties of plant hosts plays an important role in the success of management plans, so in this research the effects of five different varieties of strawberry including Queen Elisa, Parous, Gaviota, Camarosa and Selva on the biological parameters of pest was studied using the analysis method of bisexual age-specific life table in the laboratory conditions, 26±2oC, 70±5% relative humidity and 16 hours photoperiod. The obtained results showed that the length of different growth stages of the pest was significantly various for the different studied strawberry varieties. The maximum and minimum length of one generation (T) were recorded on the Parous (21.37±0.22 days) and Gaviota (16.04±1.03 days) varieties. The highest and lowest lengths of immature growth stages were obtained on the Parous (16.24± 0.83 days) and Gaviota (11.27±0.22 days). The oviposition period of the pest was also significantly different on the various hosts and the calculated values for this biological period on Queen Elisa, Parous, Gaviota, Camarosa, and Selva were recorded as 12.19±0.98, 10.09±1.02, 12.29±0.56, 13.71±1.16 and 16.61±1.34 days, respectively. In additions, the total fecundity of T. urticae was also studied on different varieties and its highest value was calculated on the Gaviota (117.93± 26.41 egg/Female). The maximum and minimum mortality rates of immature stages were obtained on the Parous (0.53±0.24%) and Selva (0.29±0.11%). The highest and lowest values of the intrinsic rate of increase (rm) were estimated on the gaviota (0.281±0.086 per day) and Parous (0.113±0.060 per day). The highest level of reproductive rate (R0) of T. urticae was calculated on the Selva, which had a significant difference with other hosts. The calculated values of the finite rate of increase (λ) of T. urticae pest was also significantly under the influence of different hosts and the maximum and minimum of this parameter were recorded on Gaviota (1.301± 0.064 per day) and Parous (1.102±0.37 per day). The results of this research demonstrate effect of different varieties on the biological parameters of two-spotted spider mite, T. urticae. Accordingly, the Gaviota and Parous varieties, respectively had the lowest and the highest negative effects on the biological parameters of T. urticae in laboratory conditions. The result of this study can be used for the management plans of the two-spotted spider mite on strawberry varieties.