In order to identify the nematode fauna of Roodghat area, Sufiyan, several soil samples were collected from rhizosphere soil of its different locations during 2016 and 2017. Nematodes were extracted, killed and fixed using commom nematological methods. Permanent slides were prepared and the extracted nematodes studied based on their morphological and morphometric characters. As a result, among the different obtained species, Doryllium zeelandicum (de Man, 1876) Loof, 1996 and Latocephalus smithi (Heyns, 1963) Patil & Khan, 1982 were also identified. According to the available literature, the both genera are new reports for Irans nematode fauna. The genus Doryllium characterized by short odontostyle, odontophore with distinct basal knobs or flanges, female genital system mono-opisthodelphic, with or without prevulval uterine sac, spicules dorylaimoid, only one ventromedian supplement and short and rounded tail. The anderstady population of showed high similarity, D. zeelandicum, but some differences such as lower head width (6-7.5 vs 8-9 μm), slightly longer odontostyle (6-9 vs 5-6 μm) and lower prerectum length (41-60 vs 70-134 μm) were also realized. It is distinguished from D. uniforme by longer body (0.78-0.90 vs 0.38-0.47 mm), greater c value (31-40 vs 21-26) and lower V value (33-37 vs 39-43). The genus Latocephalus characterized by conspicuous lateral glands and female reproductive system mono-opisthodelphic. The population of L. smithi in addition to high similarity with the species characters, some differences such as longer body size (1.22-1.37 vs 1.17-1.26 mm), greater a value (63-72 vs 53-58), lower head width (7-9 vs 9-11 μm) and shorter odontostyle (7-9 vs 9-10 μm) were found. The species L. smithi resembles to L. monhystera but it is separated from L. monhystera because of presence of prevulval uterine sac, lower b value (4.1-4.6 vs 4.7-5), greater V value (42-49 vs 38-40) and a less distance between guiding ring from anterior end (4-5 vs 6.8 μm). For the first time molecular study of the genus Latocephalus was performed using the D2-D3 region of the 28S rDNA. The sequence was compared with Discolaimoides and Carcharodiscus sequences that are available in GenBank. Phylogenetic tree was drawn, and the three genera located close to each other and formed a clade with 86% posterior probability.