Charcoal rot disease in Iran has a high geographical dispersion and there are numerous reports of its presence on different plants. In order to investigate the antagonistic power of five isolates of Streptomyces 36, 39, 46, 47 and 63 against the four isolates of Macrophomina phaseolina M0, M2, M4 and M6, two-way culture of Yuan and Crowford was used. The effect of volatile metabolites of bacterial isolates on preventing growth of fungi caused by the disease was done using the Fedamen and Dorzal method. A culture of bacterial spores with 108 cfu / ml (colony forming units per mililitr) was prepared to make the saponification culture and was spread on a plate containing potato dextrose agar (PDA). A five-millimeter disk of the young perineum of the fungus was placed in the center of the plate containing the dextrose agar apple. Both plates were placed face to face and connected to each other using parafilm. Two-way cultivars showed that 36 isolates, 46 and 47 isolates had 35, 41 and 42% inhibitory effect on mycelia growth of fungi, respectively. The antagonistic effects developed after two days and developed in the following days. There was a significant difference between isolates of 36, 46 and isolates of 36 and 47 at 5% probability level. The resuscitation test showed that after five days of incubation, the fungal mycelium, located on the margin of the reciprocal culture inhibitory zone of all five bacterial isolates, was able to grow in the new culture medium. In the volatile discharge test, all three isolates of 36, 46, and 47 could well prevent the growth of the mycelia of the pathogenic fungus. Isolates 46 and 47 with 65 and 57 percent, and isolate 36 with 50 percent inhibitory growth almost stopped the fungus colonies. Statistically, the difference in the effect of evapotranspiration in three isolates was not significant and the inhibitory effect on growth was similar between M. phaseolina isolates. This experiment was conducted in a randomized complete design with 20 treatments and 4 replications