Tomatoes from the potato family are a perennial herb that is often cultivated annually in all parts of the world. This plant is considered to be one of the most valuable vegetable and vegetable products in the Middle East, which is economically the second largest after the potato. Tomato is attacked by various pests. Among these pests can be noted different types of lepidoptera. The tomato leaf miner, Tuta absoluta (Meyrick) is a key pest of tomato in several parts of the world including Iran. Since the pest has been breeding for several generations per year, mines in the leaves, fruits, flowers, buds as well as stems. Therefore the damage is extensive and its control by pesticides makes several problems such as developing resistance. It is shown that plant growth promoting rhizobacteria (PGPRs), as useful soil micro-organisms, play an important role in sustainable agriculture. These bacteria protect plants against biological stresses, including pests, by generating siderophores, regulating nitrogen and producing plant hormones. These bacteria also protect the plant against pest infestation through Induced systemic resistance (ISR). In this study the effect of an actinobacterium, Micrococcus yunnanensis as well as their interactions on some physiological parameters on Plant and feeding of second instar larvae of tomato leaf miner under greenhouse condition were evaluated. The experiment was carried out as a factorial experiment in a complete random block design with 4 replicates per each treatment (control, bacteria). The amount of second instar larval nutrition, chlorophyll, carotenoids levels and anthocyanin were investigated. Data analysis was performed in SPSS. The results indicated that the amount of second instar larval nutrition was significantly reduced in bacteria treatments. Also chlorophyll, carotenoids levels and anthocyanin were increased in bacteria treatment. These results indicate that the Micrococcus yunnanensis bacterium increases some pigments of tomato in the presence of the second larvae of Tuta absoluta.