The fig (Ficus carica L., Moraceae) is one of the most important production in Iran and Estahban County (Fars Province) as the main center of fig production in Iran. The fig tree has always been attacked by numerous pests and one of the most important pests is fig spider mite, Eotetranychus hirsti. In this study, five common and commercial fig cultivars including “Sabz, Anjir siah, Shah anjir, Peyves and Poozdonbali” were studied and the relationship between the number of mite and the size of trichoms were estimated. For this purpose, 108 trichoms from 34 leaves were randomly selected and after cutting out, clearing and coloring with Safranin, the height and diameter of trichoms were measured under microscope with a magnification of ×40, in micrometer. Also the number of mites on the leaf of each cultivar was counted and recorded. The results showed that the height of trichoms in these five cultivars were 167.86, 188.01, 133.83, 152.05 and 109.17 µm, the diameter of the trichoms were 46.62, 54.70, 28.94, 49.51 and 34.03 µm and the means of number of mite counted on the cultivars were 55.36, 53.98, 18.20, 35.11 and 31.57 respectively. The Sabz and Anjir siah cultivars with the maximum number of mites had longer and thincker trichoms, but the Shah anjir cultivar with the minimum number of mites, had the smallest and thinnest trichoms. Finally the results showed that the cultivars with thincker and longer trichoms were sensitive and the fig with narrow and short trichoms were resistant to damage of fig spider mite. Also in this study, relative water content (RWC) of leaves was investigated, that results showed no significant difference between these cultivars.