In order to compare the agronomic characteristics and the response of important diseases of cotton in three methods and two times of cotton planting, including usual seed planting, planting without soil tillage and pot planting, in the two times, the first planting and delay planting after harvesting of wheat, the experiment was carried out in a randomized complete block design with three replications at two stations Karkandeh and Hashem Abad in Golestan province in 2013. Based on the results at Karkandeh station, showed that, plant height, first yield and total yield were significant at the 5% level in two planting date, and no significant differences were observed in other characteristics among treatments. By changing the date and method of planting, the index of verticillium wilt was not significant between the treatments, but the index of verticillium wilt in the first planting date was higher than the second plantig date after harvesting of wheat. Also this index in no-tillage method was less than two other methods. In the study of interactions between date and method of planting, the highest percentage of verticillium wilt disease was observed in first palnting date by usual planting cotton seed and the lowest percentage of verticillium wilt disease was in the first planting and no-tillage. The results in Hashem-Abad station showed that the characteristics such as the first yield, the second yield and the total yield were significant differences and were not significant in other characteristics. Yield of first palnting date was more than second planting date. Total yield of the first planting date was more than delay planting. The pot planting had the least yield and no tillage had the highest yield.