Root knot nematodes (Meloidogyne spp.) are the most important plant pathogens that cause the quantitative and qualitative damages to agriculture products. In recent years, the increasing economic importance of peach have caused using of different rootstocks in the peach gardens. GF677, (the common commercial rootstock for peach and almond) despite having the desirable characteristics, is very sensitive to root-knot nematode infection. On the other hand, the different studies have shown that the mycorrhizal fungi in addition to increase of plant growth, causing them to resistance or tolerance to plant pathogens. The similar colonization site of mycorrhizal fungi and root knot nematodes is a good reason that mycorrhizal fungi are a good option for controlling the nematodes. Investigation of interaction between Glomus mosseae and M. javanica at the different exposures times (fungus before, simultaneous and after nematode inoculation) were conducted as a completely randomized experiment with six treatments and five replications on GF677. Fungus and nematode inoculum were used as a 10% w/w of pot soil and 2000 second larvae, respectively. The results were evaluated on basis the colonization rate and mycorhizal density indices and developmental nematode characteristics (galls and egg masses number per 1 gr of root, eggs per egg mass, second larvae in 200 g of soil) four month after last nematode inoculation. The results analysis, were indicated the highest level of symbiosis (5A4) and plant growth in mycorhizal and without nematodes treatment. Although symbiosis (4A3) decreased slightly, the best effect of mycorrhiza was observed in fungus two weeks before nematode inoculation treatment. Simultaneous fungus and nematode inoculation were caused reduction of galls and nematode eggs, but due to competition, the symbiosis (2A0) decreased, so, no effect on nematode damages.