Tuta absoluta (Meyrick) is one of the pests of Solanaceae plants, especially in the world and Iran. Excessive use of chemical insecticides quickly leads to pest resistance. Using protein-based compounds such as cholesterol oxidase enzymes to control pests is a good alternative to chemical insecticides. In this research, the effect of cholesterol oxidase enzyme on the prevalence of oviposition of chinchilla mint was investigated. The three varieties including Newton, Izmir and Orgon were treated with cholesterol oxidase enzymes at 0.08, 0.2, and 0.6, mg / ml during three phenological stages 30, 45 and 60 days old plants. The experiment was carried out in 5 replicates and the oviposition rate was evaluated after 72 hours. The results showed that by increasing the enzyme concentration, oviposition decreased. Also, the highest egg number was found on the Orange variety and 0.6 mg / ml enzyme treatment. The lowest egg number was found on the Newton variety and in 0.08 mg / ml enzyme concentration and the 30-days phenology. The experiments were carried out in five replications at 25±1°C and 60±5% RH at a photoperiod of 16:8 (L: D) in greenhouse conditions. The results indicated that the cholesterol oxidase enzyme has the ability to control and deterrence effect on the pest .Also, the results so far point to the health of this compound for humans and consumer animals, because the building of this enzyme, like other proteins, is degraded and inactive under high temperature conditions and it is recommended for use in IPM in order to to protect the environment and produce chemical free products.