Fungal contamination of livestock and poultry food in most livestock and poultry in the country is one of the causes of disease and mortality of animals and chickens, in particular through the production of fungal toxins. Therefore, in order to study the fungal contamination of livestock and poultry feeds in Khorram abad, samples of dairy farms in Khorram abad were collected and transferred to the laboratory and cultured on the PDA medium. After isolation, fungi were purified and identified by morphological characteristics using valid keys and papers. To confirm the toxinization of Aspergillus and Penicillium species, a culture medium containing coconut extract and UV rays was used. A total of 150 isolates obtained species, Fusarium verticilliodes. F. proliferatum, F. equiseti. F. solani, Aspergillus flavus, A. parasiticus, A. niger, A. repens, A. aculeatus, A. carbonarius, Penicillium expansum, P. italicum, P. purpurogenum, P. islandicum, P. paxilli, P. digitatum, Alternaria solani, A. alternata, Rhizopus stolonifer, Absidia sp. were identified. Species of A. parasiticus, P. digitatum and F. verticillioides have the highest frequency (33, 24 and 20%) and A. solani had the lowest abundance (2%). As a result of this study, species of Fusarium verticilliodes, F. equiseti, A. aculeatus, A. carbonarius, P. oslonii, P. paxilli and A. alternata of livestock and poultry for the first time are reported from Iran. Also toxinization of species A. flavus, A. parasiticus, A. carbonarius, P. expansum, P. purpurogenum, P. islandicum, P. oslonii, P. paxilli and P. digitatum On the coconut agar media has been proven and the toxinization of other species is under consideration