Tetranychus urticae Koch (Acari: Tetranychidae) is the most important pest infestation in greenhouses around the world, causing significant damage to the function of many horticultural, agricultural crops and ornamental plants. Greenhouse conditions are optimum for the development of this pest and farmers are dependent on the use of chemical pesticides to control of this pest. Effects of difluvidazine (Flumite®) as a growth inhibitor and fenpropathrin (Danitol®) as a nervous irritant was investigated, on different stages of two spotted mites under laboratory conditions at 25±51ºC, 70±10 RH and 16/8 photoperiod. At the first, the most effective doses were estimated by bracketing test. Potter spray tower was used for bioassay. The results showed that difluvidazine is most effective on the stages of egg, larvae, and nymph but it doesn't have lethal effects on adults. Fenpropathrin is effective on the stages of egg, larvae, and adult but it doesn't have lethal effects on eggs. LC30 and LC50 for mites that exposed to difluvidazine are 2.218 and 3.182 ppm on egg stage, 3.292 and 5.657 ppm on larvae stage and 3.939 and 6.593 on nymph stage, respectively. Also, the toxicity of this compound on the egg stage was higher than the other stages and at the adult stage no toxicity was observed. LC30 and LC50 for mites that exposed to fenpropathrin are 20.04 and 35.518 ppm on adult stage, 3.958 and 5.655 ppm on larvae stage and 7.949 and 13.920 on nymph stage, respectively. Also, the toxicity of this compound on the larvae stage was higher than the other stages and at the egg stage no toxicity was observed.