In organic management of the soil, organic fertilizers are a suitable alternative of chemical fertilizers. Organic fertilizers have been effected to change the chemical, physical and biological properties of soil, affects the life of the soil biota including the nematodes. In present study, movement of the second-stage juveniles of root-knot nematode (Meloidogyne sp.) in columns filled with treated soil with poultry manure for 18 weeks, and untreated soil (control), under a saturated condition was investigated. Soils columns were investigated after 24 and 72 hours of nematodes injection into the soil under steady water flow conditions. The design was carried out in a randomized complete block design with factorial experiment in three replications and its results were analyzed using SAS software. The results showed that distribution of J2 was different in untreated and treated soils. The nematodes were moved at short distances from the injection point in the control treatment at 24 hours after injection and moved more distances at flow direction, also the nematodes were dispersed almost at entire the column surfaces and more moved at the flow direction, 72 hours after the injection. In the treated soil, the nematodes moved in longer distances from the injection site to the control at both of 24 and 72 hours after injection, however continued to move at the direction greater than the flow direction to the opposite direction. Evidence from this study showed that nematodes could withstand water flow, and increasing poultry manure by causing improving the physical properties of the soil, such as changing the structure and creating more porosity, would trigger a faster movement of J2 of Meloidogyne sp. in the soil. These changes can cause the larvae move away from the roots when hatching and reduce the possibility of plant contamination.