The box plant (Buxus hyrcana) in the northern Forests of Iran become frequently infested by the box tree moth, Cydalima perspectalis, over the past years, that has been identified as the most important pest of the genus Buxus in the world. Up to date applicable biological control methods for regulating of C. perspectalis in Iran is limited. In this study, the goal is to determine the LC50 values of Bio1 and Neemazal (botanical pesticide) that The Bio1 is used as a fertilizer in Iran. Experiments were performed in a completely randomized design and were evaluated on second instar larvae C. perspectalis under laboratory conditions ( 27±1 ̊C, 65±10% RH and 16h light: 8h dark). Leaf of bux plant was prepared and leaf dipping method was used for bioassays. Mortality was tested at 6 different concentrations for Bio1(1,0.7,0.5,0.3ml/L and control) and NeemAzal (1.5,1,0.5,0.25 ml/L and control) with 3 replicated and mortality was recorded after 24 and 48 h. The mortality data were subjected to probit analysis for LC50 calculation (SAS: ver.9. 0). The LC50 values of Bio1 and Neemazal were 0.165 and 0.853 ppm, respectively also the LC90 values of Bio1 and Neemazal were 0.442 and 1.52 ppm, respectively. The results indicated that both botanical pesticide have high potential for controlling of the pest but box moth was more susceptible to bio1 because of higher mortality in lower dose, On the other hand, the price of Bio1 is one third of Neemazal that become more affordable for controlling of C. perspectalis.