Abstract:
The effect of M. anisopliae against Sunn Pest’s nymphs in the field condition were evaluated in Hafshejan area near Shahr-E Kord city in 2016-2017. The test was done with M. anisopliae M14 isolate from Iranian Research Institute of Plant Protection. Experimental treatments included: 1- Naturalis®, 2.3 ×107 Spore/ml, 2- Coved spores of M. anisopliae 1 ×106 Spore/ml, 3- Granule of M. anisopliae 3 ×108 Spore/g, 4- Green Muscle®5 ×1010 Spore/g and 5- Uncovered spores (Control) of M. anisopliae 1 ×106 Spore/ml‌. Experiments were done with factorial arrangement in a completely randomized design with three replications. Analyzing variance of mortality percentage of Sunn Pest’s nymphs at 3,5,8,11,14,17, 20 and 23 days after of spore spraying was showed significant differences between years and treatments. Results of infection percentage of 10 wheat spikes of Sardari variety showed the most infection percentage at Green Muscle® and Control with 38.6± 1.44 and 36.6± 1.22% Respectively. Granule of M. anisopliae treatment with 18.8 0±0.85% and 21.6±0.15% had the lowest infection percentage in 2016 and 2017 respectively. Results showed that Granule of M. anisopliae treatment at all of sampling days introduced more mortality and Naturalis® , Covered spores of M. anisopliae , Green Muscle® and Control were in the next times. Although microbial insecticide can use for Sunn Pest control in overwintering areas as Integrated Pest Management method. Mass production of this Entomopathgen on grains such as rice, wheat and barley is achievable and it has not much cost without risk of chemical insecticides for natural enemies and environment.