Identification of root knot nematode (Meloidogyne spp.) in the tobacco fields in Gorgan and Ali Abad

Kheibali, M1. Nasrollahnejad, S2. Kavosi, K3


1- MSc. Student, Department of Plant Pathology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
(Daruosh_kh@yahoo.com)
2- Assistant Professor, Department of Plant Pathology, University of Agriculture and Natural Resources of Gorgan.
3- Assistant Professor, Department of Irrigation Engineering, Gorgan Branch, Islamic Azad University, Gorgan, Iran

Root knot nematodes (Meloidogyne spp.) are obligatory and sedentary root endoparasites of many plants, including tobacco in Iran and tobacco producing countries of the world. The genus has a wide geographical distribution and host range. They have specific and genera interactions with their host plants and other soil inhabiting pathogens. Therefore they can causea much crop loss and are considered as important diseases of plants. In this research, in order to study and to identify the species and to determine the races of root knot nematodes in the tobacco fields of Golestan province, 90 soil and root samples from tobacco fields in Gorgan and Aliabad regions were collected during 2017. Nematodes were extracted from soil (Jenkines, 1964 ( and root (Coolen & D' Herde,1972) and were counted, and species were determined by using perineal patterns obtained from adult females and reaction to differential host plans (cotton deltapin 16, tobacco NC-95, watermelon Charleston grey, tomato Rutgerse, Peanut Florunne, pepper Early California). As a result of this study, 4 species of this genus including: M. incognita Race 2, M. javanica, M. arenaria Race 2 and M. hapla were identified that M. incognita has the most plenty (79%) in comparison with others.