Cultivating resistant host plants has been considered as an important strategy for sustainable pest management of mites. The current study was conducted to evaluate the response of forty three cucumber (Cucumis sativus L.) cultivars to two important tetranychid mites, two-spotted spider mite (Tetranychus urticae Koch.) and strawberry spider mite (Tetranychus turkestani Ugarov & Nikolski). The experiment was implemented in a Randomized Complete Block design (RCB) with 43 treatments replicated 4 times in a greenhouse during 2015-2016. Two main evaluation criteria, cultivar injury rate (from 1 to 5) and number of eggs/female/day (EFD) on each cultivar, were used to compare responses. The data were analyzed using SAS, 9.1 software and mean comparisons were performed by LSD test. The results revealed that the highest FED number for both mites was observed on Taha cultivar (9.0±0.40 and 9.5±0.28 for T. urticae and T. turkestani, respectively). The highest injury rate was also observed on this cultivar (4.8±0.26 and 5.0±0.0 for T. urticae and T. turkestani, respectively). In contrast, the lowest EFD number for T. urticae (2.8±0.47) and T. turkestani (2.5±0.28) was seen on Samer star and Davos cultivars, respectively. However, no host preference was shown by T. turkestai in the two Iver and Samer star cultivars for egg laying. These two cultivars had the lowest injury level too (1.2±0.25 for Samer star and 1.5±0.28 for Negeen to T. urticae and T. turkestani, respectively). Our data indicate that Taha is a susceptible cultivar and Samer star and Negeen are resistant cultivars. We recommend the use of these two cultivars for resistance to spider mites under greenhouse conditions.