The two-spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) is a general feeder that attacks a wide variety of plants. It is a highly polyphagous pest and is considered as an economic importance for many horticultural and field crops. its short life cycle, abundant progeny and arrhenotokous reproduction, it is able to develop resistance to chemical compounds very rapidly. As a consequence, it has the dubious reputation to be the“most resistant species” in terms of the total number of pesticides to which populations have become resistant, and its control has become problematic .in many areas worldwide. Botanical insecticides have become increasingly relevant in the realm of pest control. In this study, we examined phospholipid fractions of four oil seeds including, soybean Glycine max cultivar Gk, rapeseed Brassica napus cultivar Hayola, linseed Linum usitatissimum cultivar Kazakhstan and cotton Gossypium hirsutum cultivar Golestan meal on Tetranychus urticae. Experiments were carried out at 25±1 ºC, 70±5% RH and photoperiod of 16:8 (L: D) h. The experiments were performed by dipping method with three replications. Adult mortality was assessed 24 h after exposure, and the females were considered dead if unable to move across a distance at least equal to their body length. Results showed that all formulated compounds had lethal effects. The percent mortality was increased as concentration increased. The LC50 values of the phospholipid fractions of soybean, rapeseed, linseed and cotton were 2680, 2800, 3100 and 3800 ppm, respectively. All compounds were toxic to the mite, and the values were not significantly different. In conclusion, phospholipid fractions of the studied oil seeds were found to be a suitable alternative for controlling the two-spotted spider mite