More than 200 specimens of agaric Fungi were collected form Ghalajeh heights and surrounding plains, located in Kermanshah Province, western Iran. Sampling were conducted from 2014- 2017, The specimens were identified based on macroscopic characteristics (including shape and dimensions of pileus and stipe, the presence or absence of volva, gill attachment, the spore print color, etc.) and microscopic features (the presence or absence of clamp connection, shape and type of hypha; shape, color and size of basidiospore, shape, size and position of cystidia, shape and number of srigmata, etc.). After preliminary investigation, the specimens were dried using a dehydrator. In order to remove any possible pests which may have escaped from the effects of the drying process, the packets containing dried specimens were placed in a freezer at -20°C for at least one week and then they were kept in zip-locked plastic bags. A specimen from each identified species collected from different geographical region was deposited in the herbarium of fungi, Iranian Research Institute of Plant Protection, Tehran, Iran. Then, the internal transcribed spacer sequences of the selected specimens were analyzed to confirm the morphological identification. Based on the morphological, and phylogenetic examinations, fifty four species belonging to 24 genera of agaric Fungi were identified. Among the identified species, 32 species are reported for the first time from Kermanshah Province and 18 species including Amanita lividopallescens, Conocybe velutinomarginata, Coprinopsis insignis, Coprinopsis semitalis, Coprinus pinetorum, Entoloma phaeocyathus, Entoloma undulatosporum, Lepista sordida, Panaeolus guttulatus, Panaeolus olivaceus, Pluteus cinerofuscus, Pogonoloma macrocephala, Psathyrella fatua, Psathyrella leucotephra, Psathyrella longicauda, Psathyrella senex, Saproamanta vitadinii and Tulostoma cretaceum are new for the mycobiota of Iran.