The plants from the point of view food supply for human, animals, processing of industrial and Medicinal products and healthy environment are so important. About 90% of human food provided from plants. So, maintaining the health of the plants involve of particular importance. In Iran, there is no precise information from the amount of disease damage. Although, they know amount of it is about 15%. The Nanotechnology provides developing area in all fields of human life and biology and especially medical and biotechnology and have main role in improving product management techniques. Afew studies have done about Antibacterial effects of Nano materials on plant pathogenic bacteria. Thus in this study, the effects of nanoparticles on bacteria was investigated. The silver nanoparticles without increasing drug resistance caused the inhibition of the bacteries respiratory system and also silver in dimensions of Nano affecting on the metabolism and microorganism reproduction. This element has been including of specific properties in microbial decontamination. In this research the antibacterial effect have investigated on the six different levels of silver Nanocid ( 1, 5, 50, 100, 200, 400 ppm) and four type antibiotics (Amoxicillin, erythromycin, tetracycline, penicillin) on five strain bacteria (Agrobacterium, Pseudomonas, Xanthomonas, Bacillus, Rhodococcus). In this research the trend was ascending antibacterial activity increased by increasing the density of nanocid. In all five bacterial strains, the most affected in Rhodococcus and Bacillus bacteria, which is Gram-positive, also had more effect. Nanocide at a concentration up of 200 ppm in all bacteria other than Agrobacterium, the effect of nanocid is more than antibiotics. So, the results showed that by increasing the density of silver nanocide, the amount of the antibacterial effect increases in most bacteria, to the extent that even the antibiotics will have the same or superior result.