Response study of biological control agents to pesticides could be instrumental for increasing the efficacy and success of integrated pest management programs. One of the well-known biological control agents is Amblyseius swirskii (Athias-Henriot) a predator mite, which plays an important role in controlling of many important pests such as mites, thrips and whiteflies. Here, attempts were made to investigate the sub-lethal effects of a new pesticide, Spirotetramat (Movento®), on the biological parameters of adult stage of this predator in laboratory conditions (25±2 °C, 65±5% RH, photoperiod of 16 L). Tetramic acid derivative Spirotetramat, a recently introduced ambimobile compound, has shown an outstanding performance against sucking pest. First, Tetranychus urticae Koch and A. swirskii were mass-reared on cucumber. Then Spirotetramat LC50 assayed on T. urticae protonymph stage through exposing them to leaves of a previously sprayed plant and consequently, sub-lethal doses (LC10, LC20 and LC30) calculated as it follows; 55.8, 100.2 and 152.7 ppm, respectively. Next, A. swirskii were allowed to feed on poisoned mites with sub-lethal doses and four biological parameters including Predatory, adult Longevity, oviposition period and fecundity of adult stage monitored in two consecutive generations. Data were analyzed based on the Age-Stage Two Sex Life Table theory. The aforementioned parameters for LC30 treatment determined 5.66 prey per day, 30.98 day, 28.95 day and 25.24 in first generation and 5.67 prey per day, 30.89 day, 27.61 day and 25.79 in second generation, respectively, which didn’t have significant difference with other treatments (LC10, LC20) and control in both generation. In all treatments net reproductive rate (R0), the intrinsic (r) and finite (λ) rate of population increase and mean of generation time (T) was calculated, and their amount in LC30 treatment was as it follows; 15.78 offspring per female, 0.164 day-1, 1.17 day-1 and 16.48, respectively. No significant differences were observed among all treatments which implies integration of A. swirskii and Spirotetramat could be advisable.