The tomato plant, known as Solanum lycopersicum that is one of the native plants of South and Central America that has been transported to other countries throughout the Spanish colonial period. First Fusarium disease report as crown and root rot disease with factor Fusarium oxysporum f.sp. radicis-lycopersici Jarvis & Shoemaker (FORL) from Japan and then from other parts of the world. The fungus causing rotting of the crown and root of tomato is one of the most important and destructive diseases of the soil born in farms and greenhouses. The disease can be greatly expanded and severely reduce the quality and quantity of the product in farms and greenhouses. The rhizosphere microorganisms, due to the suitable control power, environmental health and human is a good option for use in disease management and biological control programs. In the rhizosphere, the first root defensive line of the plant against soil pathogens is the useful soil microorganisms. The rhizosphere is a plant rich in microbial agents and colonizing rhizobacteria. Actinobacteria produce many secondary metabolites with biological activity. In this study, bacteria were identified after isolation by biochemical, morphological and nucleotide sequences with 16s rDNA gene. The isolates of UTS629 and UTS632 showed the highest inhibitory effect on this fungus growth, and these two did not show significant differences based on the Duncan test at 5% level, and the isolate UTS1345 had the low control. Greenhouse experiments started in one of the greenhouses of the Department of Plant Protection of the Agriculture & Natural resources Campus of the University of Tehran by planting early urbana tomato seeds from July 2017 and continued until the end of August 2017. In this experiment isolates UTS529, UTS2000 and UTS629 showed the most control effect.