Wheat and barley are the country's strategic crops and are more cultivated than other crops. Given that global demand for cereals is steadily increasing, the continued increase in their production is crucial for food supplies. The genus Bipolaris includes a number of significant plant pathogens. These species are commonly associated with leaf spots, leaf blights, melting outs, root rots, foot rots and other disease symptoms mainly in high value field crops in the family Poaceae. With regard to the importance of plant diseases and economic damage, as well as the undeniable role of cereals in the food basket of people, in order to protect plants against pathogens, there are various measures that use chemical poisons of one Of these. Considering the environmental hazards of the use of chemical pesticides, the integrated management of the disease and the replacement or prioritization of safe and hazardous methods, such as the use of biocontrol agents, is of great importance. Among these biocontrol agents are Trichoderma species. In this study, the antagonistic relationship between isolates of and fungal isolates of Bipolaris spp. Intercropping, as well as the effect of volatile compounds of Trichoderma isolates, were studied on the pathogenic fungi. For this purpose, the three-day culture of three-day cultivar of three-day isolates of six strains of Trichoderma T.harzianum, T.orientalis, T. koningii, T.viridae, T.arundinaceum, T.atroviridae, 5 mm was cut and on The PDA environment was cultivated against each other. After reaching each other, the ability to colonize and prevent colonization of pathogenic agents was measured and analyzed. In the study of volatile compounds, Trichoderma fungi and pathogens were placed on the PDA. Two petty pancakes were joined together by cellophane and after 96 hours, the colonization prevention rate was measured and calculated. In the intercropping test, T.harzianum isolates and T.atroviridae isolates with 30 and 28.92 percent showed the highest inhibitory effect in controlling the disease. In the test of volatile matter, T. koningii and T.viridae isolates were 61.80% and 53.93%, respectively, in the comparison of the mean values of the growth factor inhibitory growth of the fungus causing the most effect on the control of the disease agent and potentiality of becoming commercially effective isolates.