Strawberries are always at the risk of lots of fungal infection, but Botrytis cinerea and Rhizopus stolonifer are the most important disease factors. On the other hand, managing strawberries decay factors by using chemical fungicides, doesn’t seem the most appropriate way because strawberries are used fresh and there is always the risk of toxicity. So, controlling biologic factors as a solution is under study all over the world. This study is concerned with the effect of controlling some artificial, chemical, physical and biological factors on 2 fungal infections: B. cinerea and R. stolonifer. For this reason, 12 isolates of Botrytis and 9 isolates of Rhizopus were collected from some infected fruit to soft and gray decay in Kurdistan, Tehran and Alborz. After purification, isolates' disease was proved by using common ways.Then, isolates' morphological characterization was performed based on vegetative characteristics and decreasing the growth of fungi was done by biologic ways accompanied with Pichia Rodotorula, Guilliermondii, Mucilaginosa and Saccharomyces cerevisiae yeast species. After purification, the pathogenicity of the isolates was confirmed using common methods. Morphological identification of isolates was carried out using vegetative characteristics. , based on the result of N2, O2, CO2 and vacant assessment on isolates' growth, CO2 has the most effect on decreasing growth (86.25%) and N2 has the least effect (5.25%). The results of this study showed that the physical method used in this study has a significant effect on controlling the soft and gray rot of strawberry and with more reviews and studies, better results can be achieved.