Strawberries are always at the risk of lots of fungal infection, but Botrytis cinerea and Rhizopus stolonifer are the most important disease factors. On the other hand, managing strawberries decay factors by using chemical fungicides, doesn’t seem the most appropriate way because strawberries are used fresh and there is always the risk of toxicity. So, controlling biologic factors as a solution is under study all over the world. This study is concerned with the effect of controlling some artificial, chemical, physical and biological factors on 2 fungal infections: B. cinerea and R. stolonifer. For this reason, 12 isolates of Botrytis and 9 isolates of Rhizopus were collected from some infected fruit to soft and gray decay in Kurdistan, Tehran and Alborz. After purification, isolates' disease was proved by using common ways.Then, isolates' morphological characterization was performed based on vegetative characteristics and decreasing the growth of fungi was done by biologic ways accompanied with Pichia Rodotorula, Guilliermondii, Mucilaginosa and Saccharomyces cerevisiae yeast species. The results of the evaluation of Sodium Bicarbonate and Chloride Calcium assessment on isolated samples growth signified Sodium Bicarbonate with 2 percent and more density nearly 100% stop Mycelium’s growth.and chloride-calcium was significant at 4% concentration. The results of this study showed that the use of sodium and calcium bicarbonate in this study has a significant effect on the control of soft and gray rot of strawberry, the present study, considering the existing information, has significant effect on controlling soft and gray decay of strawberries by using chemical, physical and biological ways. Thus, further investigation will be resulted better result.