Garlic rust disease, Puccinia porri and or P. allii, is one of the major diseases in garlic fields in the world and Iran. In this research, the distribution of the garlic and the susceptibility of the garlic genotypes to rust disease have been carried out. The identification of the disease cause, confirms Puccinia allii, which was reported before and has been occurred severly in garlic growing areas in Isfahan province at present. The only uridinal stages along with the uridiospores were observed on the infected leaves. The disease was also obseverd on leek plants, and not on the other ones In the survey in Isfahan garlic growing areas, 10 samples per diameter from every garlic fields in the different areas including: Fereidoonshahr, Frieden, Semirom, Isfahan, Asfhank, Falavarjan, North and South, as well as Zeyar were collected randomly and the percent infection and the disease severity were determined accordingly. In this regards the development of disease progression along with the disease severity at the six indices of 0, 10, 25, 50, 75 and 100 were assessed. Results revealed that the growth trend had a direct correlation with the percentage of infection and disease severit. So that, the parameter sometimes had reached the highest level as irrigation and suitable condition was prevailed. Also, the assessment of disease severity on the leaf surface indicated that the highest and lowest severity of the factor with 87.5 percent and 80 were associated to Lenjan and Pyrbkran respectively. These results indicating the importance of the rust disease in garlic growing zones in Isfahan province too. The combined response of the garlic genotypes to rust disease progression showed that, the genotype Sholan with 30.81 percent disease severity was the only susceptible genotype, whereas the genotypes of Touin and Schourin with 21.68 and 21.48percent severity respectively. Aliabad genotypes with 13 persent and then Bahar with 16.37 were the lowest ones in the groups with statistically significant effects were tested separately with other genotypes. The remaining two spectra are intermediate genotypes and statistically significant effect related groups have been located.