Two-spotted spider mite (Tetranychus urticae) is global spread and causes economic damage on many plants, including cucumbers. Generally, farmers use acaricide to control it. In order to diversify and prevent the resistance of this pest to miticide, a study was conducted to evaluate on the effectiveness of the newest acaricide acequinocyl (Kanemite SC 15%) in Hormozgan and Yazd provinces. Mode of action of this acaricide is mitochondrial complex III electron transport inhibitors. Field studies were conducted in randomized complete block design with 5 treatments including Kane mite SC 15% with 1, 1.25 ml/L, tetradifon and spiromsifen (Oberon SC 24) with 2 and 0.5 ml/L respectively, and Plain water was used as control. Sampling of two-spotted spider (Tetranychus urticae) population performed at one day before and 3, 7, 14 days after spraying. The result of this research in 7 days after spraying in Hormozgan and yazd for Kanemite SC 15% with 1.25 and 1 ml/L, tetradifon and spirodiclofen (Oberon) with 2 and 0.5 ml/L showed, 85-84, 64-76, 95-88 and 80-85 mortality percentage respectively. Mean comparing showed Kanemite 1.25 ml/L, tetradifon and Oberon with 2 and 0.5 ml/L had no significant different and the more efficacy than Kanemite 1 ml/L. Based on the results, it was found that the Kanemite in a concentration of 1.25 ml/L in both regains is acceptable and can be recommended.