Pistachio is one of the most important products of the garden, which is known as the Iranian name and its production in our country has a historical record. One of the most important problems in Iran's pistachio areas is soil salinity, which causes a lot of nutritional problems for pistachio trees. In this study, the effects of different levels of Pseudomonas PGPR (R8 from Pseudomonas putida and R115 isolate from P. fluorescens) and Mycorrhiza fungi (one level of Myco Root, a mixture of three species Glomus mosea, G. etunicatum and G. intraradices) on three pistachio cultivars including Badami Zarand, Akbari and Ahmad Aghaei were studied in a saline soil, as a factorial experiment in a completely randomized design. The results showed that combined treatment of mycorhiza and bacterial isolates increased the chlorophyll content by 49.83%, 42.12% and 19.6%, respectively, for Badami, Akbari and Ahmad Ahagai cultivars compared to the control. After co-administration of mycorrhizal fungi and PGPR isolate of R8, the highest increase in leaf chlorophyll content was observed in R153+mycorrhiza treatment, 31.5%, 30.12% and 25.25%, respectively for Badami Akbari and Ahmad Ahaghi cultivars, respectively compared to the control. After two treatments, mycorrhizal fungi, isolate B1 bacteria and then isolate B2 bacteria alone increased the chlorophyll content leaves. For growth character, the stem diameter increase was similar to the increase of chlorophyll content and the highest stem diameter was observed in the Mycorrhiza fungi + R8 isolate bacteria, the diameter stem for Badami, Akbari and Ahmad Ahaghi cultivarswas observed, respectively, 3.30 (control 1.90 mm), 3.05 (control 47.1 mm) and 2.84 (control 1.45 mm). Based on the results of this research, it can be concluded that the use of mycorrhiza and PGPR bacteria in saline soils can have an effect on plant physiology, help the plant growth and improve the growth and physiological characteristics.