Vegetable leaf miner, Liriomyza sativae Blanchard, is one of the most important and destructive greenhouse cucumber pests in South and Central America and Europe that caused heavy damage to greenhouses and cucumber fields. This pest is one of the most important pests of greenhouse cucumbers in Iran. Since the use of resistant cultivars is considered as one of the effective on IPM program, the tolerance of the seven cultivars of greenhouse cucumbers (Khasib, Radiante, Caspian, Nisim, Storm, Janet and Afzal) to vegetable leaf miner were examined in greenhouse condition with temperature of 27 ± 2 ° C, relative humidity of 60 ± 5% and an light period of 16 hours of light and 8 hours of darkness. Decrease the plant height, the plant growth rate, the shortness rate and the fresh weight and the dry weight loss of cultivars were compared with control cultivars in a randomized complete block design. Analysis of variance of the data showed a significant difference between studied cultivars. Comparison of meanings showed that the lowest decrease plant height was observed in Afzal cultivar with mean of 36.2% and the highest decrease plant height was observed in Nasim cultivar with mean of 42.75%. The highest and the lowest percentage of infected plant growth comparing to the control were observed in Afzal and Nasim cultivars with means of 63.98% and 57.25%, respectively. The lowest shortness rate of the infected plant comparing to the control was observed in Afzal and Caspian cultivars with means of 13.42 and 12.16 percent and the highest amount was observed in Nasim with mean of 16.14 percent, respectively. The lowest fresh weight loss in infected plants comparing to the control was observed in Afzal cultivar with mean of 8.92% and the highest were observed in Nasim and Storm cultivars with means of 33.49% and 28.16%, respectively. The lowest dry weight loss in infected plants comparing to the control were observed in Afzal and Janet cultivars with means of 38.94 and 35.32 percent and the highest dry weight loss were observed in Storm and Nasim cultivars with means of 64.53 and 67.33 percent, respectively. It seems some cucumber cultivars have a good effect on tolerance of vegetable leaf miner.