معرفی دو گونه جدید از خانواده Longidoridae Thorne, 1935 از منطقه رودقات- صوفیان، استان آذربایجان شرقی

Roodghat- Sufiyan located 40 kilometers far away northwest of Tabriz covering approximately 100 hectares area which has not been studied for its nematode fauna. During a survey on plant parasitic and free living nematodes in this region, several soil samples were collected during 2016 and 2017 and processed at the Nematology Laboratory, University of Tabriz, Tabriz, Iran. Nematodes were extracted using tray method, killed and fixed following De Grisse method. Temporary and permanent microscopic slides of the nematodes were prepared and studied based on morphological and morphometric characters. Among the recovered nematodes, two species of plant parasitic nematodes Longidorus sp. and Xiphinema sp. belonging to the family Longidoridae were identified. Longidorus sp., can be characterised by having an anteriorly flatted continuous head region, pocket-shaped not bilobed amphids, guiding ring typical of the genus, tail short and bluntly rounded, four juvenile stages, absence of males and arrangement of the oesophageal glands so that, the dorsal nucleus is located at about the middle of the expanded part of esophagus and is larger than the subventral nuclei, the latters are placed in the posterior quarter of the expanded part, belongs to the L. closelongatus group. Longidorus sp. can be differentiated from L. cretensis by its shorter odontostyle (105-114 vs 128-146 μm), anteriorly positioned guiding ring (34-38 vs 42-47 μm) and characters of juveniles. Xiphinema sp. belongs to group 6 of intraspecies grouping sensu Loof and Luc, 1990 and characterized by having two almost equally developed female reproductive branches with spines in the tubular part of the uterus, body length 3.67-4.25 mm, spear 215-225 μm long, tail short, dorsally convex, presence of blind canal with a peg in the end, three juvenile stages and absence of males. Xiphinema sp. is separated from X. vuittenezi because of longer body (3.67-4.25 vs 2.75-3.92 mm), greater c (103-133 vs 72-96), less c′ (0.7-0.9 vs 0.9-1.2) and anteriorly located vulva (V= 46-48 vs 48-52), longer spear (215-225 vs 188-208 μm) and characters of juveniles. For both the species, molecular analyzes were also performed for more accuracy. For molecular study, DNA of the nematodes extracted and D2-D3 of 28S rDNA was amplified and sequenced. This amplified region consists of 800 bps length approximately. The results of the phylogenetic analyses were found supportive of specifies status. In this study, X. index, X. pachtaicum and X. vuittenezi were also collected and identified.