گزارش یک گونه جدید و یک گونه شناخته شده از جنس Aporcella Andrassy, 2002از ایران

In a survey conducted to identify nematode fauna in Roodghat area, Sufiyan, East Azarbaijan province, several soil samples were collected from the rhizosphere of different vegetation of the region during 2016 and 2017. The soil samples were processed and the nematodes extracted using modified Jenkins method (centrifugal-flotation method). The nematodes were killed, fixed and transferred to anhydrous glycerin according to De Grisse method. Permanent microscopic slides were prepared from the nematodes. Then, their morphological and morphometrical characteristics were evaluated using light microscopy and a drawing tube. Among the extracted nematodes, the genus Aporcella belonging to Aporcelaimidae family was identified with one un-described Aporcella sp. and one known species Aporcella capitulum. According to available literature, the genus Aporcella is a new report for Iran’s nematode fauna. The genus Aporcella is charactertized by location of S1N1 at about middle of DN-S1N2 distance and absence of pars refringens vaginae. The new species differs from A. chardimensis because of its shorter body (1.05-1.15 vs 1.38-1.77 mm), lower c value (33-36 vs 46-69), greater c′ (1.4-1.6 vs 0.8-1.1) and less lip region width (11-13 vs 16-17 μm). After comparing the morphological and morphological data of Iranian population of Aporcella capitulum with the original description, in addition to high similarites with this description, some differences including b (3.7-4.4 vs 4.4-5.6) and c (37-47 vs 44-64) values, lip region width (15-16 vs 17-20 μm) and the presence of multiple glands in the lateral cords were found, too. Aporcella capitulum is comparable to A. adriaani. It is separated from A. adriaani because of greater body length (1.66-2.02 vs 1.23-1.49 mm), c′ value (1.1-1.4 vs 0.8-1.1), lip region width (15-16 vs 12-15 μm) and tail length (40-47 vs 21-30 μm). In order to more accuracy the D2-D3 region of 28S rDNA gene was amplified with the relevant primers. The amplified region has approximately 800 bps length. The phylogenetic tree was drawn using the homologous sequences obtained from the GenBank. The above species, combined with A. simplex and A. chardimensis were included in a group with 97% posterior probability. The studied species, along with A. chardimensis formed a sister group with A. simplex. The phylogenetic analyses also revealed the relationship of the genera Carcharodiscus, Discolaimoides and Discolaimus belonging to Qudsianematidae family to the genus Aporcella.