معرفی گونه های جدید قارچی از گیاهان زینتی در استان آذربایجان غربی

During 2016-2017, ornamental plants in greenhouses were visited in West Azerbaijan province, and many symptomatic (blight and wilting) plant specimens were collected. Plant samples were transferred to the mycology lab in clean paper bags and processed immediately. Plant specimens were subjected to surface sterilization with 1% sodium hypochlorite sodium solution for 1-2 min then rinsed three times by sterile water. Fungi isolation was done by using a blotter method, and purified cultures were yielded by transferring a single spore produced on plant tissues onto 2% water agar (2% WA) and subsequently a hyphal tip onto potato dextrose agar (PDA). Purified isolates were maintained on PDA slants at 4 °C for future examinations. All of isolates were identified using morphological and molecular data (ITS-rDNA, GPD (glyceraldehyde-3-phosphate dehydrogenase gene), TEF-1a (translation elongation factor-1a gene) and beta-Tubulin genes). The sequence comparisons were done in NCBI BLAST and QBank nucleotide search tools to determine the similar sequences and to molecular identification of the given isolates. Six fungal species identified on ornamental plants including Fusarium cortaderia (on Beaucarnea recurvate and Phoenix canariensis plants), Allophoma labilis (on Begonia semperflorens), Neosetophoma samarorum (on Ficus elastica), Botrytis cinerea (on Pelargonium graveolens, Pelargonium Hortorum, Aquilegia vulgaris, Agave americana, Ficus benjamina and Impatiens hawker plants), Stemphylium lycopersici (on Cyperus alternifolius), Colletotrichum siamense (on Schefflera arboricola), Colletotrichum clivicola (on Ficus benjamina) and Bipolaris oryzae (on Cycas revoluta). Species of Fusarium cortaderia, Allophoma labilis, Neosetophoma samarorum, and Stemphylium lycopersici are new fungal species for Iranian mycobiota. Further studies are needed to determine the distribution and to assess the economic impacts of theses species in West Azerbaijan province.