گزارش تجمع پروتئاز تریتیسن آلفا گندم در روده حشرات کامل سن گندم با استفاده از تکنیک پروتئومیکس

Using of proteomic technique as powerful method to identify Physiological proteins of insects is new way in insect Physiology. Digestive system of the hemiptera consists from the salivary gland and alimentary canal. Proteome patterns of salivary gland and gut in the adult sunn pest and fifth- instar nymphs were determined with two dimensional electrophoresis by authors. The study of insect digestive system particularly sunn pest causes new potential targets in pest management. Any disruption in the normal process of insect nutrition can be reduced or suppressed feeding and assimilation in the insects. In this study, recurrent sunn pest after 2- days feeding were selected for dissection. Gut proteins visualized by two dimensional electrophoresis patterns. There were many spots in the proteome patterns that need to be identified. One of the main spots that not identified but exist in the all of replication was selected as target protein. This spot cut and digest with trypsin and then injected to liquid chromatography and mass spectrometry ( LC/ MS-MS). After analysis of resulted spectrums using National center of biotechnology information with two enteries Animals and wheat, target protein identifies as triticin-α of wheat. This result showed that accumulated plant protease in the gut of sunn pest can be used as a source of amino acids in the nutrition process. Although some of wheat proteins were identified by the authors (like serpin, beta amylase and dehydro ascorbat reductase) in the gut of sunn pest, but supplementary studies are necessary to detect effective proteins before and after feeding and before and after different treatments.