گزارش چند گونه نماتد جنس Tylenchorhynchus (Dolichodoridae) از ایران

In order to identify the plant parasitic nematodes of the family Dolichodoridae in northern and north-western provinces of Iran, 610 soil and root samples from agricultural and natural habitats in East Azarbaijan, West Azarbaijan, Ardabil, Zanjan, Semnan, Qazvin, Golestan, Gilan and Mazandaran provinces were collected during 2012- 2015. After extraction, killing and fixation of nematodes by transferred to glycerin according to the modified De Grisse method, identification of specieses based on morphological and morphometrical characters was performed using the light microscope, equipped with digital, camera. And the use of SSU and LSU gene for molecular study. At finally 7 species belonging to Tylenchorhynchus were identified as following T. clarus, T. dubius, Tylenchorhynchus sp., T. maximus, T. microconus, T. parvus and T. serranus, two species Tylenchorhynchus microconus with Lip region rounded, offset from body by 3-4 distinct annuli, cephalic framework lightly sclerotized outer extension extending over 1-2 body annuli , basal knobs prominent, rounded, with sloping laterally, Tail conoid, bluntly rounded with 10-15 annuli on ventral side and tail terminus smooth, Phasmids conspicuous, 25-28 μm behind anus and Tylenchorhynchus serranus with 4 lines at lateral field, outer bands areolated, Lip region hemispherical, anteriorly flattened, set off by a slight constriction from the body by 6-8 distinct annuli, cephalic framework lightly sclerotized outer extension extending over 2-3 body annuli, Fasciculi present, Short post-anal intestinal sac,Tail subcylindroid, with rounded terminus, with 42-48 annuli on ventral side and tail terminus striated, this population have male with 14-15 μm length of spicule, are new records from Iran.