میزبان های طبیعی ویروس پیچیدگی برگ کنجد یک تِرنکِرتوویروس جدید از مزارع کنجد استان کرمان

The genus Turncurtovirus have a unique genome organization and is one of the nine genera in the family Geminiviridae. Turncurtoviruses are transmitted by the Circulifer haematoceps (Cicadellidae) in a persistent and circulative manner. Turnip curly top virus (TCTV) is the only definitive member of the genus which was first reported in turnip growing farms in Fars province in 2010. Recently, a new turncurtovirus was recovered from C. haematoceps collected from Orzoeiyeh, Jiroft and Sirjan (Kerman province) using combination of rolling circle amplification (RCA) and PCR, however, no natural plant host has been already identified for the virus. To detect the virus in natural plant hosts, leaf samples of different crops showing leaf curling with cup-shaped, malformation and yellowing symptoms were collected in Jiroft, Bam and Kerman and total DNA was extracted using CTAB method. The viral infection of the samples was tested by PCR using specific primer pair and 1500 bp in length amplicon was detected for sesame, faba bean, spinach, bean, parsley, radish and alfalfa. Full-length genome of a sesame isolate was amplified using RCA method, cloned and sequenced. Nucleotide sequence comparison showed that the sesame isolate of the virus is most similar to TCTV in GenBank (% 65.5 nucleotide identity). Therefore, the recovered virus from leafhopper is a new member of the genus Turncurtovirus and due to distribution of the virus in sesame farms the putative name sesame curly top virus was provisionally proposed. According to the results of this study, turncurtoviruses have a high genetic diversity in Iran.