استفاده از ژن میتوکندری CO1 در شناسایی گونه‌ی کفشدوزک آسیایی، Harmonia axyridis (Pallas) (Col.: Coccinellidae), : نخستین بار در ایران

The Asian ladybird beetle, Harmonia axyridis (Pallas) (Col.: Coccinellidae), is one of the most invasive insects worldwide which has been introduced into several continents for the classical biological control. Thus, analysis of mitochondrial DNA provides important information about the genetic diversity of spreading populations. The current study is the first report of this species in Iran. Sampling different morphs of H. axyridis were collected from fruit and ornamental trees in Rasht, Guilan Province, north Iran during 2016– 2017. All fifteen morphs and different stages of eggs, larvae, pupae and adults of H. axyridis specimens were collected on common hazel (Corylus avellana L.), wild plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.) trees, crepe myrtle (Lagerstroemia indica (L.) Pers.) and oleander (Nerium oleander L.). For genus and species identification of Asian ladybird beetle was used proliferation and cytochrome c oxidase (COI) gene sequencing method. DNA was extracted from whole specimens using the DNPTM Kit (#DN8115C) CinnaGen. Sequencing of the obtained 560 bp product, such as CTAAGGAATG AGAGTGTGCT CCATGTACTA CCCATGACCA CGATTAAATA ACATAAGATT TTAGACTTTT ACCCCCTGCT TTAACTCTTT TAATTTTAAG AACAATCGTA GAAATAGGGG CAGGAACAGG ATGAACTGTT TACCCTCCTC TTTCTTCTAA TTTAACACAT AATGGGCCTT CAGTAGATTT AGTGATTTTT AGTTTACATT TAGCAGGAAT TTCCTCAATT TTAGGTGCAG TAAATTTCAT TTCAACTATT ATAAATATAC GTCCATTTGG TATAATACTT GATAAAACTC CTTTATTTGT ATGATCTGTT CTTATTACAG CAATTCTTTT ATTACTATCA CTACCAGTTC TTGCAGGAGC AATTACTATA CTATTAACTG ACCGAAACTT AAATTCTTCT TTTTTTGACC CAACCGGTGG GGGAGACCCA ATTTTATACC AACATTTATT TTGATTTTTT GGCCATCCTG AAGTTTATAT TTTATTTTTA CCGGGA. Blast search of COI sequences of our specimens revealed high similarity (99%) of the amplified region with COI reported in GenBank (NCBI) from native and invasive populations of H. axyridis in all over the world (China, USA, Canada, Germany, Lithuania). Thus, fifteen slightly different aberrations of two major colour morphs (succinea, spectabilis) were similar as in other invasive populations of H. axyridis.