شناسایی عامل پوسیدگی نرم باکتریایی سیر در جنوب کرمان

Garlic (Allium sativum) is a member of the Alliaceae family. This plant has been widely recognized as a valuable spice and a popular remedy for various ailments and physiological disorders. It is a herbaceous plant which is one of the most important economic bulb crops in Iran. Iran, with an annual production of more than 100,000 tons of garlic, has ranked number 13 in the world. Kerman province is one of the most important producers of this crop and the major areas of garlic cultivation in the province include Shahdad and southern Kerman and its high quality. From southern Kerman garlic fields, a number of some sample suspected bacterial pathogens with symptoms of leaf spot and soft rot on garlic bulbs were collected. Plant materials after surface-sterilization were cultured on NA, EMB and King, B and some strains of bacteria were isolated from these samples. Colonies were non-florescent on KB, white to colorless on NA and emerald green on EMB. In terms the biochemical and physiological characterization of these strains were gram-negative, rod shaped, facultative anaerobe, oxidase and arginine dihydrolase negative, catalase and hyper-sensitivity reaction on Geranium positive and were able rotted potato tuber slices. Genotype detection with three pairs of ADE1 / ADE2, EXPCCF / EXPCCR and Ecal / Eca2 specific primers were showed that three isolates belonged to the Dickeya chrysanthemi species. The results of the pathogenicity test showed that three isolates can cause symptoms of soft rot in the garlic plant. Based on our knowledge this is the first report of soft rot on Garlic in Iran caused by Dickeya chrysanthemi.