شیوع ویروس بادنا -1 انجیر در استان فارس

Abstract
Fig mosaic occurs worldwide and is the most common and important viral disease of fig. Although fig mosaic disease (FMD) has been recorded in Iran since some years ago, its high current incidence in fig orchards of Fars province, as one of the main fig production regions, alarms for better understanding of the viral complex associated with the disease. In the quest to identify the causal agent of the disease, several new viruses have been identified, including a new DNA virus from genus Badnavirus. The infected fig-trees showed different types of mosaic, chlorotic spots, ring spots ,malformation of leaves and necrotic symptoms. To unravel the virus or virus complex involved in FMD in Fars province, possible presence of Fig badnavirus-1 [FBV-1 (genus Badnavirus, family Caulimoviridae)] in infected trees of this region was assessed by PCR. Twenty leaf samples from five fig orchards were randomly collected and tested for the presence of FBV-1 using total DNA extracted from leaf samples (Dellaporta et al., 1983) and the primer pair 580F/1650R, described by Laney et al. (2012). Eighteen out of the twenty tested samples proved to be infected with FBV-1, as shown by amplification of a 1070 bp DNA fragment encompassing ORF1, ORF2 and ORF3 of the viral genome. The complete nucleotide sequence of FBV-1 was then determined by PCR using virus specific primers. The viral genome comprises 7140 nucleotides. It has a deduced genome organization typical for a member of the genus Badnavirus and the complete nucleotide sequence of FBV-1 analysis showed 99% nucleotide sequence homology with isolates from Arkansas (USA) and other isolates previously reported from other parts of IRAN.