بررسی مقاومت پاتوتیپ‌های A و A* باکتری Xanthomonas citri subsp. citri به برخی از ترکیبات مسی

In recent decades, copper's antimicrobial compounds has been used to control a wide range of bacterial diseases. Resistance to these compounds in Target-Pathogen species and reduced disease control is a common problem due to the frequent use of copper bacteria. Resistance to copper compounds has been observed in many plant pathogenic bacteria such as Pseudomonas, Pantoea, Erwinia and various species of Xanthomonas. Six strains of pathotypes A and A* were used in order to investigate resistance to copper compounds in laboratory conditions. In this study, growth of bacteria in Mannitol-Glutamate Yeast (MGY) with three copper compounds of Bordeauxfix, Copprer oxychloride and copper sulfate with concentrations of 0.1, 0.3, 0.6, 1.2 and 2.4 mM were investigated. Six strains tested were grown at a concentration of 0.1 mM of Bordeauxfix, Copprer oxychloride and copper sulfate. Strains of M11 and S14 were grown at 1.2 mM Bordeauxfix, strains S14 and B17 were grown at 1.2 mM Copprer oxychloride and strains B17, A7 and S14 were grown at 1.2mM copper sulfate, and only one strain of S14, was grown at all concentrations of the three chemicals. In order to investigate the effect of copper compounds in growth chamber conditions, two strains S14 and LMG9322 (strain type) were inoculated into lime leaf, and then that copper compounds were sprayed at a concentration of 1.2 mM. The populations of these strains were measured at intervals of 1, 4, 7, 10, 12 and 20 days after spraying of chemicals. Although two strains (S14 and LMG9322) inoculated into the lime leaves showed more increase in the population of bacteria and pathogenicity in compared to those treated with Bordeauxfix and Copprer oxychloride, but in leaves treated with three copper compounds, growth of bacterial and pathogenic populations were observed in leaves inoculated with S14, which shows that there are copper-resistant genes in this isolate.