القای پروتئین‌های PR توسط اسید سالیسیلیک و جاسمونیک در گوجه‌فرنگی(Solanum lycopersicum cv. Superchief) مایه‌زنی شده با ویروس موزاییک خیار (CMV)

Induction of resistance to Cucumber mosaic virus (CMV) in tomato plants (cv. Superchief) by application of salicylic acid (SA) and jasmonic acid (JA) was evaluated in this experiment. Treatments included CMV, SA (1 mM), JA (0.5 mM), SA+JA, SA+CMV, JA+CMV, SA+JA+CMV and a control. CMV was mechanically inoculated on tomato plants using carborundum. Samples were taken at 0, 1, 2, 4, 6, 8, and 15 days post inoculation (dpi). Based on the results, photosynthesis rate and chlorophyll a and b content decreased under the virus infection and hormone treatment. SA increased both chlorophyll a and b contents in comparison to the control over the time, but JA only increased the chlorophyll a content and had a negative effect on chlorophyll b content. Expression of CMV coat protein gene (CMV-CP) was decreased approximately 48% in SA+JA+CMV treatment in comparison to the CMV- infected plants at 15 dpi. The control and hormone-treated without CMV plants had no detectable CMV-CP gene. The highest expression rate of PR1 and PR10 observed in SA+JA+CMV treatment that were 28 and 24% greater than CMV at 6 and 8 dpi, respectively. Expression of PR1 was greater in treatments consisting CMV in comparison to hormone treatments without CMV. Expression of PR10 was the highest in SA+JA+CMV at 8 dpi and reduced at 15 dpi. Tomato plants dry weight decreased in comparison to control in all treatments. The highest dry weight loss (47%) observed in JA+CMV that was not significantly different with CMV (44%). In conclusion, combination of SA and JA improved the resistance against CMV in tomato (cv. Superchief) and increased aerial dry weight about 22% compared with CMV control.