شناسایی عوامل باکتریایی پوسیدگی نرم و ساق سیاه سیب زمینی در استان کرمان

Kerman provice has the fifth rank in the production and supply of potatoes in the country. Inorder to identify the factors ofpotato soft rot, the samples with suspected symptoms were isolated and purified on bacteriological culture media from storehouse during the years 1394-1395. Single colonies were studied for continuation of the research. Totally 64 bacterial isolates were isolated from the pathogenicity test. Pectolytic isolates were evaluated in physiological, morphological and biochemical characteristics based on conventional bacteriological methods. Phenotypic characteristics of pectolytic bacteria isolated from isolates of Pectobacterium carotovorum subsp.carotovorum, Pectobacterium atrosepticum and Dickeya chrysanthemi species were studied. For the purpose of molecular analysis, specific primers were used to indicate the three pectic species Pectobacterium atrosepticum, Pectobacterium carotovorum subsp. carotovorum, Dickeya chrysanthemi. Comparison of the results with standard health samples confirmed the results. The highest infection rate in Pectobacterium carotovorum subsp.carotovorum in Faryab region was 85% and the lowest infection was in Jiroft region with 12% contamination with Dickeya chrysanthemi. The highest percentage of distribution of Pectobacterium atrosepticum in Lalezar region with 95% was in Manojan and the tissues without this species. Dickeya chrysanthemi had the highest distribution in Manojan region with 53.33%. This species was not isolated from LalehZar area. Also, Pectobacterium carotovorum subsp.carotovorum has a maximum distribution of 46.66% in Manojan region and has the lowest distribution in Lalezar and Jiroft areas, in proportion to 7.4% compared to the sampling areas. Totally, the results indicated geographical location and climate conditions of sampling areas with distribution percentage and percentage contamination of pectolytic species.