جداسازی و شناسایی باکتری Microbacterium sp. از گیاه سیب زمینی در استان کرمان

Potato (Sulanum tubersum L.) is one of the most important agricultural products that plays an important role for feeding many people in the world and also in industrial applications .Samples were taken from different areas of potato cultivation in Kerman province during 1394-1395, then, these samples were transferred into the plant pathology laboratory. At first the plant tissues were washed with ordinary water and after disinfection with 70% alcohol in petri dishes containing sterilized distilled water, they were crushed into the small pieces. After 20 minutes, the suspensions were dipped on a Nutrient agar medium containing 1% sucrose (NAS). And then incubated for 3-4 days at 25- 25 Celsius degrees. The colonies were isolated and purified on common culture media of bacteriology. By performing PCR and using the (FD1 / RD1) primer pairs, a piece with a length of approximately 1100 base pairs was propagated. Propagation and sequencing was determined by Topaz gene Research company and the sequences were compared using the Blast n program with standard strains comparison in the NCBI. This isolate has the ability to create an inhibitory shade on a culture medium containing Pectobacterium atrosepticum colonies. The antagonistic potential of the isolate was also studied in greenhouse conditions. Based on the nucleotide sequence, the sample was 99% similar to Microbacterium sp. and Also, the ability to create an inhibitory halo (Antibiosis) on a culture medium containing Pectobacterium atrosepticum colonies The donor is an antagonistic feature of the isolate studied. This is the first report for the presence of endophyte bacteria Microbacterium sp. on potatoes from Iran.