ردیابی سرولوژیکی و مولکولی ویروس موزاییک معمولی بافت مرده لوبیا (BCMNV) از مناطق لوبیا کاری شهرستان ارومیه

Legumes (Leguminosae) are the most important food source after cereals and among them, beans are one of the most important sources of protein and energy for humans. Diseases in the world reduce more than 10% of total bean production. Among these pathogens, there are many viruses that harmful to beans, one of the most important of these viral diseases is Bean common mosaic necrosis virus (BCMNV). The virus is from the Potyviridae family and the Potyvirus genus. In order to detection and identification of Bean common mosaic necrosis virus in Urmia bean Fields, plants with viral symptoms were collected during the 2018 growing season and the location and date of sampling registered on special plastic bags individually. Symptoms that were observed on the bean fields included yellowing, malformation, curly top, mosaics, mottling and stunting. Collected samples were analyzed by DAS-ELISA test using BCMNV specific antibody. Based on the results of ELISA, The 10 of the tested samples had positive reaction with specific virus antibodies. These samples were subjected to complementary study using reverse transcription polymerase chain reaction (RT-PCR) and using specific primers for coat protein of Bean common mosaic necrosis virus. The results showed the amplification of a DNA fragment with an expected length (900 bp) in 6 samples. Analysis of the obtained sequences using the BLAST tools in NCBI web site and it comparison with available sequences in GenBank showed 99% similarity with the previously reported sequences about BCMNV from other parts of the world. The results of taxonomical research will be determined in the future.