قارچ‎های اندوفیت همزیست با گیاه دارویی ‏Ferula flabelliloba‏ جمع آوری شده از ارتفاعات زشک استان ‏خراسان رضوی

Ferula spp., belong to Umbelliferae family, are medicinal plants which are mostly found in Mediterranean area and ‎Central Asia. Several species of this genus have been used in folk medicine for their antispasmodic, carminative, ‎digestive, expectorant, sedative, antihysteric, laxative, aphrodisiac, antiseptic ana analgestic activities. Their ‎endophytic fungi have not been studied in the world, yet. Endophytic fungi associated with medicinal plants have ‎the potential to produce new secondary metabolites, due to long-term coexistence with these plants. On the other ‎hand, using the fungi to make these valuable compounds is much easier and more affordable. So, in this study, we ‎tried to isolate and identify fungal symbiotic species of Ferula ovina collected from Zoshk hights of Khorasan ‎Razavi province. Pieces of 0.5-1 cm from the root tissue were cultured on PDA containing streptomycin (20 µg/ml) ‎after washing with sterile distilled water and three steps disinfection with 70% ethanol and 0.5% sodium ‎hypochlorite. Then, they were incubated at 25-30‎‏ ‏‎°C for 7 to 14 days. Grown fungi were transfered into the new ‎plates containing WA media and puriffied. After DNA extraction of isolates, ITS, LSU and Ef1α regions were ‎amplified, sequenced and compared to the sequences of other fungi in GeneBank. Sequences of all three replicated ‎regions were recorded in the GeneBank and accession numbers received. Acording to morphological and ‎molecoular identifications isolates were belong to 7 different genera which Muriphaesphaeria sp. nov. and ‎Cadophora sp. nov. are first reports for the world. Other species were belonged to Leucosphaerina arxii,‎‏ ‏Alternaria ‎clamydosporigena, Rhizoctonia solani, Myrothecium sp. and two Fusarium species, F. solani and F. avenaceum.‎‏ ‏