معرفی برخی قارچ های همراه با علایم لکه برگی در گیاه همیشک (Danae racemosa)

Danae racemosa is a small shrub belonging to Liliaceae family with a height of 50 to 100 cm, evergreen plant and a medicinal ornament. Todays, the green parts of this plant and herbal tea of it, are used as a medicinal plant in traditional medicine. During march 2018, in order to identification of fungi associated with leaf spots symptoms, some infected leaf samples were collected from Danae racemosa shrub situated at the College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. The infected leaf samples were transferred to mycology laboratory and diseased parts of leaves were cut in to pieces, then surface sterilized with 1% sodium hypochlorite solution and finally were washed with sterile water and placed on 2% water agar (WA) culture medium. In addition, the diseased leaf samples were investigated in blotter test with putting the samples on the moisture filter papers. The petri dishes were kept at room temperature for several days. Purification of fungal isolates was done using single spore and hyphal tip methods on potato dextrose agar (PDA) medium. Fungal colonies were grown at 25°C for seven days under alternate light condition with 12 hr light/12 hr darkness period. Based on morphological characteristics of the fungal isolates and using related mycological keys, some taxa belonging to Ulocladium, Alternaria, Cladosporium, Phoma, Pestaloshiopsis and Coniothyrium genera were identified. The isolates of these genera is under consideration for identificain at the species level. All the above-mentioned fungi are reported for the first time from Danae racemosa in Iran.