شناسایی قارچ‌های اندوفیت گیاهان Glaucium fimbrilligerum، Chelidonium majusو Papaver macrostomum از تیره خشخاش در استان گلستان

Endophytes are fungi that live within plants for at least a part of their life cycle without causing any visible manifestation of disease. Studies have shown that, nearly 300,000 plants species that exist on the earth, each individual plant is the host to one or more endophytes. Many endophytes have the potential to synthesize various bioactive metabolites. Many researches have proven that endophyte is new and potential source of novel natural products for exploitation in modern medicine, agriculture and industry. In order to identify the endophytic fungi from Papaver macrostomum, Chelidonium majus and Glaucium fimbrilligerum samples were taken from node of healthy plants from different locations in Gorgan, during the summer and autumn of 2017. Samples were surface sterilized with 70% ethanol (v/v) for 45 second, 1% sodium hypochlorite (v/v) for 30 second, and then washed three times with sterile distilled water. Samples were plated on potato dextrose agar culture medium and incubated at 25°C for 4 weeks. During this time, the fungi growing out of samples were isolated and purified by single spore and hyphal tip methods. Each isolate was identified by morphological characteristics such as conidia and conidiophore using appropriate identification keys. In order to identify non-spore isolates and verify the authenticity of the findings based on morphological characteristics, molecular identification and sequencing of ITS regions (ITS4 and ITS5) from the ribosomal DNA were performed and compared with GeneBank. In this study, 3 fungal species including Alternaria japonica, Alternaria arborescens and Lopadostoma fagi were identified as endophytic fungi of Papaveraceae. Alternaria species and a new genus reported for the first time form plants family of papaveraceae.