اولین گزارش از تعدادی بیمارگر قارچی روی گیاه توت فرنگی از جنوب استان کرمان

Fungi causing disease on crown and root of strawberry are varied and belonging to various genera and species. As regards to variable weather condition in each region, combination of the fungal genera and species are also different. In order to identify fungal pathogens of root and crown of strawberry in south of Kerman province, samples with crown and root rot and wilting symptoms collected. Isolation and purification of fungi were done in culture media. Microscopic slides prepared from fungi growing in culture medium in lactic acid 50%. Morphological characters of fungal structures were examined using light microscopy. A combination of morphological features including conidiomata (if present), conidiogenous loci, shape, size and septation of conidia and conidiophores have been used to identify the taxa. Extraction of genomic DNA performed based on CTAB method. Quality and quantity and of extracted DNA investigated through agarose gel electrophoresis and spectrophotometry. Then ITS region of the ribosomal DNA amplified using ITS1 and ITS4 primers and PCR products were sequenced. Sequences of taxa compared with other correlate and similar sequences in Gene Bank. With morphological data and nucleotide sequencing of ITS-rDNA region, three species of the genus Fusarium including F. chlamydosporum, F. oxysporum, F. solani and some other taxa including Bionectria ochroleuea, Clonostachys rosea, Macrophomina phaseolina and Rhizoctonia solani were identified from crown and root rot and wilting symptoms of strawberry. Pathogenicity test of Fusarium, Macrophomona and Rhizoctonia isolates on strawberry seedlings were positive and the same primary crown and root rot and wilting symptoms observed on healthy seedlings. This is the first report of Clonostachys rosea (Teleomorph: Bionectria ochroleuea) and Fusarium chlamydosporum on strawberry in Iran.