اولین گزارش از Bursaphelenchus hofmanni (Nematoda: Aphelenchoididae) در ایران

During a study that was conducted to identify the plant parasitic nematodes of Abhar township, samples from soil, root and aerial tissues of plants were collected from different crops during 2015. Nematodes were extracted and processed to anhydrous glycerin by using common methods. Permanent slides were prepared and nematodes were studied based on morphological and morphometrics characters. Among the examined species, a population of the genus Bursaphelenchus was detected and identified to B. hofmanni Braasch, 1998 that is new for nematode fauna of Iran. This species could be separated from closely related species by a relatively small body length, females and males averaging 577and 462µm, respectively, the a-value in females 27.7(24.1-30.8), with a stylet length of 12(11.5-13) µm. The male have relatively small and slightly curved spicules (length 12.7(11.5-14) µm) with a prominent rostrum and a small cucullus. The female tail is conoid and curved with finely rounded terminus. The females have a small vulval flap and some morphological characters. For molecular study of the Iranian population of B. hofmanni, a part of small subunit rDNA segment of 18S rRNA was amplified and sequenced. The obtained sequence (KY471647) was compared with closely related species sequences based on blastN search in GenBank and showed 100% identity with other sequences of B. hofmanni (KF496907, AY508018). Inferred trees based on molecular phylogenetic analysis of the small subunit of ribosomal DNA, placed B. hofmanni in a monophyletic clade with B. pinasteri (AM397016) and a sisiter group with B. parapinasteri (KT878515) and B. mazandaranense (JN153102) with 100% support.