ارزیابی اثر القای مقاومت به بیماری سپتوریوز برگی و افزایش میزان رشد گیاه گندم با استفاده از قارچ اندوفیت Piriformospora indica و باکتری Pseudomonas protegens

Septoria leaf blotch is one of the most important disease in wheat. Today, the use of biological agents to increase the amount of growth and induction of resistance in plants has been considered by researchers. In this study, after sampling from Golestan province field, the agent cultured and purified. To investigate the resistance induction, Tajan sensitive variety and P.indica and P.protogens biocontrol agents used. After preparing suspension of endophytic fungi at a concentration of 105 spores per liter and bacteria with 109 cells per ml, germinated wheat seeds, they were placed on a shaker for 12 hours at 90 rpm. The seeds were cultured in 15 cm diameter pots. The culture media included soil, sand and perlite in the same amount and autoclaved for two hours at 1/5 atmospheres. This experiment was conducted in a completely randomized design with three replications. Treatments including plant treatment by P.indica, treatments with bacteria P.protogens and the combined treatment of the plant with P.indica and P.protogens bacteria. The control treatment was considered as planting without a biological agent. Irrigation through sub pot mat and temperature was set at about 23˚C. After 21 days, the symbiosis of two biological agents in the root of the plant investigated. A 5-day suspension of Z.tritici with a concentration of 107 spores per liter was sprayed onto plants. The pots were placed at 21 ° C and saturated humidity for up to 72 hours. After that, moisture content of over 70% was provided for up to 18 days. After 21 days, the percentage of pycnidia content of leaf area and resistance of plants was calculated by Zhang et al. (2001). At the end of vegetative growth, differences in dry and wet weight, root and stem length and number of pycnidia in leaf spot were determined. According to the results of this research, Treated plants by P.indica were moderately resistant, treated plants by P.protogens and combined treated plants by P.indica and P.protogens were resistant. Control treatment plants were also sensitive. Plants treated with P.protogens, It has the highest stem and root length and also has the highest amount of dry and wet root and stem and had a significant difference at 95%. Plants treated with P.indica had fewer number of pycnidia than other treatments and their pycnidia were smaller in diameter.